Platelet and Red Blood Cell Biology and Physiology

The metabolic profiles of platelets (e.g., glycolysis rate) and RBC (e.g., lipid turnover) suggest there may be important targets that could be exploited to improve the storage characteristics and ultimately clinical efficacy of these products. Describing these profiles and relating them to in vivo outcomes remains an intense area of investigation and discovery. Modified methods of storage (e.g., anaerobic) or treatment (e.g., RBC rejuvenation) affect these profiles. Examination of these novel treatment methods will provide important clues to ultimate efficacy outcomes. We will continue to examine these associations in available databases, evaluate novel methods of collection/treatment, and relate these to in vivo outcomes. These efforts are in collaboration with several investigative partners.

Selected Publications:

D’Alessandro A, Gray A., Sczcepiorkowski ZM, Hansen K, Herschel LH, Dumont LJ. Red Blood Cell Metabolic Responses to Refrigerated Storage, Rejuvenation and Frozen Storage. Transfusion 2017 (in press); 10 MAR 2017 | DOI: 10.1111/trf.14034

de Wolski K, Fu X, Dumont LJ, Roback JD, Waterman H, Odem-Davis K, Howie HL, Zimring JC. Metabolic pathways that correlate with post-transfusion circulation of stored murine red blood cells. Haematologica. 2016;101:578-86. PMID: 26921359

Burns JM, Yoshida T, Dumont LJ, Yang X, Piety NZ, Shevkoplyas SS. Deterioration of red blood cell mechanical properties is reduced in anaerobic storage. Blood Transfus. 2016;14:80-8. PMID: 26674833

Nemkov T, Hansen KC, Dumont LJ, D'Alessandro A. Metabolomics in transfusion medicine. Transfusion. 2016;56:980-93. PMID: 26662506

Dumont LJ, D'Alessandro A, Szczepiorkowski ZM, Yoshida T. CO2 –dependent metabolic modulation in red blood cells stored under anaerobic conditions. Transfusion. 2016;56:392-403. PMID: 26477888

D’Alessandro A, Nemkov T, Hansen KC, Szczepiorkowski ZM, Dumont LJ. Red blood cell storage in additive solution-7 preserves energy and redox metabolism: a metabolomics approach. Transfusion 2015;55:2955-66. PMID: 26271632.

Roback JD, Josephson CD, Waller EK, Newman JL, Karatela S, Uppal K, Jones DP, Zimring JC, Dumont LJ. Metabolomics of ADSOL (AS-1) red blood cell storage.Transfus Med Rev. 2014;28:41-55. PMID: 24636780

Dumont LJ. Cancelas JA*, Graminske S, Friedman KD, Vassallo RR, Whitley PH, Rugg N, Dumont DF, Herschel L, Siegal AH, Szczepiorkowski ZM, Fender L, Razatos A. In vitro and in vivo quality of leukocyte-reduced apheresis platelets stored in a new platelet additive solution. Transfusion 2013;53:972-80. PMID: 22882530